mcherry-p62 delta PB1 silent
(Plasmid #187985)

• Purpose: human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552
• Depositing Lab: Sascha Martens
• Publication: Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.

Backbone

• Vector backbone: pmCherry-C1 Vector
• Backbone manufacturer: Clontech #632524
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 5707
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p62
• Species: H. sapiens (human)
• Mutation: Lacking PB1 domain (aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon)
• GenBank ID: 23636 NM_153719
• Entrez Gene: NUP62 (a.k.a. IBSN, SNDI, p62)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AAAAGAGTGCCGGCGGGACCACCGCCCACCGTGTGCTCA
• 3′ sequencing primer: TCACAACGGCGGGGGATGCTTTGAATACTGGATGG

Resource Information

• Supplemental Documents:
  • SMc552_mCherry-p62 sil 103-440 (dPB1).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry