mCherry-p62-LIR-mutant (LIRAAAA)
(Plasmid #187981)

• Purpose: Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528
• Depositing Lab: Sascha Martens
• Publication: Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.

Backbone

• Vector backbone: pmCherry-C1 Vector
• Backbone manufacturer: Clontech #632524
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 6013
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p62
• Species: H. sapiens (human)
• Insert Size (bp): 1324
• Mutation: DDDW335-338AAAA (LIR mutant), silent mutation siRNA5 BS
• GenBank ID: 23636 NM_153719
• Entrez Gene: NUP62 (a.k.a. IBSN, SNDI, p62)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CAAATGGCGTCGCTCACCGTGAAGGCCTACCT
• 3′ sequencing primer: GGTCACAACGGCGGGGGATGCTTTGAATACTG

Resource Information

• Supplemental Documents:
  • SMc528_mCherry-p62 3DW sil.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry