pWZL Blast Snail ER
(Plasmid #18798)

• Depositing Lab: Bob Weinberg
• Publication: Mani et al Cell. 2008 May 16. 133(4):704-15.

Backbone

• Vector backbone: pWZL-Blast
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: snail
• Species: H. sapiens (human)
• Mutation: G525R mutation in the ER. The presence of the G525R point mutation in the cloned ER sequence abrogates its binding to 17β-estradiol while keeping its full sensitivity to the synthetic ligand 4'hydroxytamoxifen (4OHT).
• Entrez Gene: SNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
• Tag / Fusion Protein:
  • ER hormone binding domain (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: IRES-R (5'-GCATTCCTTTGGCGAGAG-3')

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pWZL-Blast-Snail-ER was
generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER
(Littlewood et al., 1995; Watnick et al., 2003) with the hSnail cDNA PCR amplified from pBp-hSnail.