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pET28a-VcRibB
(Plasmid #187977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5587
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VcRibB
  • Alt name
    3,4-dihydroxy-2-butanone 4-phosphate synthase
  • Alt name
    RibB
  • Species
    Vibrio cholerae
  • Insert Size (bp)
    654
  • GenBank ID
    WP_001076646.1
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer TAG TTA TTG CTC AGC GGT GG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was codon optimized, synthesized and cloned into pET28a by Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-VcRibB was a gift from Audrey Lamb (Addgene plasmid # 187977 ; http://n2t.net/addgene:187977 ; RRID:Addgene_187977)

  • For your References section:

    Evidence for the Chemical Mechanism of RibB (3,4-Dihydroxy-2-butanone 4-phosphate Synthase) of Riboflavin Biosynthesis. Kenjic N, Meneely KM, Wherritt DJ, Denler MC, Jackson TA, Moran GR, Lamb AL. J Am Chem Soc. 2022 Jul 20;144(28):12769-12780. doi: 10.1021/jacs.2c03376. Epub 2022 Jul 8. 10.1021/jacs.2c03376 PubMed 35802469
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