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Addgene

pET15TV-L-AldA WT
(Plasmid #187976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15TV-L
  • Backbone manufacturer
    Cheryl Arrowsmith
  • Backbone size w/o insert (bp) 7746
  • Total vector size (bp) 8838
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AldA WT
  • Alt name
    human aldolase A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1092
  • GenBank ID
    CAG46678.1
  • Entrez Gene
    ALDOA (a.k.a. ALDA, GSD12, HEL-S-87p)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer TAG TTA TTG CTC AGC GGT GG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was codon optimized and synthesized into pET15TV-L by Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15TV-L-AldA WT was a gift from Audrey Lamb (Addgene plasmid # 187976 ; http://n2t.net/addgene:187976 ; RRID:Addgene_187976)

  • For your References section:

    Substitutions at a rheostat position in human aldolase A cause a shift in the conformational population. Fenton KD, Meneely KM, Wu T, Martin TA, Swint-Kruse L, Fenton AW, Lamb AL. Protein Sci. 2022 Feb;31(2):357-370. doi: 10.1002/pro.4222. Epub 2021 Nov 12. 10.1002/pro.4222 PubMed 34734672
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