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Addgene

pET15TV-L-hlPYK WT
(Plasmid #187975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15TV-L
  • Backbone manufacturer
    Cheryl Arrowsmith
  • Backbone size w/o insert (bp) 7746
  • Total vector size (bp) 9375
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hlPYK WT
  • Alt name
    human liver pyruvate kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1629
  • GenBank ID
    P30613.2
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer TAG TTA TTG CTC AGC GGT GG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was codon optimized and synthesized by Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15TV-L-hlPYK WT was a gift from Audrey Lamb (Addgene plasmid # 187975 ; http://n2t.net/addgene:187975 ; RRID:Addgene_187975)

  • For your References section:

    Changes in the allosteric site of human liver pyruvate kinase upon activator binding include the breakage of an intersubunit cation-pi bond. McFarlane JS, Ronnebaum TA, Meneely KM, Chilton A, Fenton AW, Lamb AL. Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):461-469. doi: 10.1107/S2053230X19007209. Epub 2019 Jun 10. 10.1107/S2053230X19007209 PubMed 31204694
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