pET29b-pvdF WT
(Plasmid #187974)

• Purpose: Expresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosa
• Depositing Lab: Audrey Lamb
• Publication: Kenjic et al Arch Biochem Biophys. 2019 Mar 30;664:40-50. doi: 10.1016/j.abb.2019.01.028. Epub 2019 Jan 26.

Backbone

• Vector backbone: pET29b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 6195
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pvdF WT
• Alt name: hydroxyornithine transformylase
• Species: Pseudomonas aeruginosa PAO1
• Insert Size (bp): 825
• GenBank ID: WP_003114501.1
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: ATG CGT CCG GCG TAG A
• 3′ sequencing primer: TAG TTA TTG CTC AGC GGT GG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry