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Addgene

pET29b-pvdF WT
(Plasmid #187974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 6195
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pvdF WT
  • Alt name
    hydroxyornithine transformylase
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    825
  • GenBank ID
    WP_003114501.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer TAG TTA TTG CTC AGC GGT GG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-pvdF WT was a gift from Audrey Lamb (Addgene plasmid # 187974 ; http://n2t.net/addgene:187974 ; RRID:Addgene_187974)

  • For your References section:

    PvdF of pyoverdin biosynthesis is a structurally unique N(10)-formyltetrahydrofolate-dependent formyltransferase. Kenjic N, Hoag MR, Moraski GC, Caperelli CA, Moran GR, Lamb AL. Arch Biochem Biophys. 2019 Mar 30;664:40-50. doi: 10.1016/j.abb.2019.01.028. Epub 2019 Jan 26. 10.1016/j.abb.2019.01.028 PubMed 30689984
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