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Addgene

pET28b-pchD WT
(Plasmid #187973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 7012
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pchD WT
  • Alt name
    salicylate adenylase
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    1644
  • GenBank ID
    WP_058181173.1
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHi (not destroyed)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer TAG TTA TTG CTC AGC GGT GG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-pchD WT was a gift from Audrey Lamb (Addgene plasmid # 187973 ; http://n2t.net/addgene:187973 ; RRID:Addgene_187973)

  • For your References section:

    Rational inhibitor design for Pseudomonas aeruginosa salicylate adenylation enzyme PchD. Shelton CL, Meneely KM, Ronnebaum TA, Chilton AS, Riley AP, Prisinzano TE, Lamb AL. J Biol Inorg Chem. 2022 May 5. pii: 10.1007/s00775-022-01941-8. doi: 10.1007/s00775-022-01941-8. 10.1007/s00775-022-01941-8 PubMed 35513576
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