pYZ960
(Plasmid #187972)

• Purpose: TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3
• Depositing Lab: Jef Boeke
• Publication: Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491.

Backbone

• Vector backbone: pRS426
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 12431
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: 30°C is highly recommended for this large plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 (codon optimized for human)
• gRNA/shRNA sequence: -
• Species: S. cerevisiae (budding yeast)
• Promoter: TEF1
• Tag / Fusion Protein:
  • SV40 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13Rev
• 3′ sequencing primer: M13Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cas9 expression module sub-cloned from Addgene #43802

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry