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Addgene

pYZ463
(Plasmid #187971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 10865
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    30°C is highly recommended for this large plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 (codon optimized for human)
  • gRNA/shRNA sequence
    -
  • Species
    S. cerevisiae (budding yeast)
  • Promoter TEF1
  • Tag / Fusion Protein
    • SV40 (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cas9 expression module sub-cloned from Addgene #43802

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYZ463 was a gift from Jef Boeke (Addgene plasmid # 187971 ; http://n2t.net/addgene:187971 ; RRID:Addgene_187971)

  • For your References section:

    CREEPY: CRISPR-mediated editing of synthetic episomes in yeast. Zhao Y, Coelho C, Lauer S, Majewski M, Laurent JM, Brosh R, Boeke JD. Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491. 10.1093/nar/gkad491 PubMed 37326023
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