pYZ462
(Plasmid
#187970)
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PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
- Backbone size w/o insert (bp) 4898
- Total vector size (bp) 10037
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructions30°C is highly recommended for this large plasmid.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9 (codon optimized for human)
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gRNA/shRNA sequence-
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SpeciesS. cerevisiae (budding yeast)
- Promoter TEF1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13Rev
- 3′ sequencing primer M13Fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCas9 expression module sub-cloned from Addgene #43802
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYZ462 was a gift from Jef Boeke (Addgene plasmid # 187970 ; http://n2t.net/addgene:187970 ; RRID:Addgene_187970) -
For your References section:
CREEPY: CRISPR-mediated editing of synthetic episomes in yeast. Zhao Y, Coelho C, Lauer S, Majewski M, Laurent JM, Brosh R, Boeke JD. Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491. 10.1093/nar/gkad491 PubMed 37326023