RFP-FKBP-U2AF35
(Plasmid #187969)

• Purpose: expresses U2AF35 with N-terminal tags RFP and FKBP
• Depositing Lab: Markus Wahl
• Publication: Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y.

Backbone

• Vector backbone: pRFP-FKBP
• Backbone size w/o insert (bp): 5010
• Total vector size (bp): 5730
• Vector type: Mammalian Expression ; CMV Promoter
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U2AF35
• Species: M. musculus (mouse)
• Insert Size (bp): 720
• GenBank ID: NM_024187
• Entrez Gene: U2af1 (a.k.a. 2010107D16Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP-FKBP (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-for (Microsynth)
• 3′ sequencing primer: FastBac-rev (Microsynth)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Heyd, FU Berlin

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry