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Addgene

RFP-FKBP-PRPF38A
(Plasmid #187968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRFP-FKBP
  • Backbone size w/o insert (bp) 4989
  • Total vector size (bp) 5946
  • Vector type
    Mammalian Expression ; CMV Promoter
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prp38A
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    957
  • GenBank ID
    NM_032864
  • Entrez Gene
    PRPF38A (a.k.a. PRP38A, Prp38)
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP-FKBP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-for (Microsynth)
  • 3′ sequencing primer FastBac-rev (Microsynth)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RFP-FKBP-PRPF38A was a gift from Markus Wahl (Addgene plasmid # 187968 ; http://n2t.net/addgene:187968 ; RRID:Addgene_187968)

  • For your References section:

    Recruitment of a splicing factor to the nuclear lamina for its inactivation. Vester K, Preussner M, Holton N, Feng S, Schultz C, Heyd F, Wahl MC. Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y. 10.1038/s42003-022-03689-y PubMed 35869234
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