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Addgene

HP1-ECFP-SNAP
(Plasmid #187966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-SNAP
  • Backbone manufacturer
    Labor Prof. Carsten Schultz, EMBL Heidelberg
  • Backbone size w/o insert (bp) 6601
  • Total vector size (bp) 7169
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HP1
  • Alt name
    CBX5, HP1A, HEL25
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    568
  • GenBank ID
    NM_012117.3 NM_012117.3
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • ECFP-SNAP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-for (Microsynth)
  • 3′ sequencing primer EGFP-N-rev (Microsynth)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ordered from Addgene No17652

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HP1-ECFP-SNAP was a gift from Markus Wahl (Addgene plasmid # 187966 ; http://n2t.net/addgene:187966 ; RRID:Addgene_187966)

  • For your References section:

    Recruitment of a splicing factor to the nuclear lamina for its inactivation. Vester K, Preussner M, Holton N, Feng S, Schultz C, Heyd F, Wahl MC. Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y. 10.1038/s42003-022-03689-y PubMed 35869234
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