Skip to main content
Addgene

Mito-SBFP2
(Plasmid #187964)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mito
  • Alt name
    Mitochondrial targeting signal from COX8A
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004074.2
  • Entrez Gene
    COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
  • Promoter CMV
  • Tag / Fusion Protein
    • SBFP2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-ACGGCAAGCTGACCCTGAAG
  • 3′ sequencing primer 5'-GGCATGGACGAGCTGTACAAGTAATAGCGGCCGCAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Recloned this from DsRed2-Mito.
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mito-SBFP2 was a gift from Erika Holzbaur (Addgene plasmid # 187964 ; http://n2t.net/addgene:187964 ; RRID:Addgene_187964)

  • For your References section:

    Damaged mitochondria recruit the effector NEMO to activate NF-kappaB signaling. Harding O, Holzer E, Riley JF, Martens S, Holzbaur ELF. Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005. 10.1016/j.molcel.2023.08.005 PubMed 37683611
Related items: