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pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
(Plasmid #187960)

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Item Catalog # Description Quantity Price (USD)
Plasmid 187960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-OsTIR1
  • Backbone size w/o insert (bp) 11406
  • Total vector size (bp) 11776
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
  • Alt name
    S. pyogenes Cas9 (D10A; N863A)
  • Alt name
    Auxin inducible degron (AID)
  • Species
    A. thaliana (mustard weed), Synthetic; S. pyogenes
  • Insert Size (bp)
    2360
  • Tag / Fusion Protein
    • GGS linker (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The AID degron domain was amplified from pcDNA5-H2B-AID-EYFP, a gift from Don Cleveland (Addgene plasmid # 47329 ; http://n2t.net/addgene:47329 ; RRID:Addgene_47329). The OsTIR1 protein employed for the AID and mAID degron systems was amplified from pMK232 (CMV-OsTIR1-PURO), which was a gift from Masato Kanemaki (Addgene plasmid # 72834 ; http://n2t.net/addgene:72834 ; RRID:Addgene_72834)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1 was a gift from Edda Schulz (Addgene plasmid # 187960 ; http://n2t.net/addgene:187960 ; RRID:Addgene_187960)

  • For your References section:

    CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Noviello G, Gjaltema RAF, Schulz EG. Nat Commun. 2023 Jun 3;14(1):3225. doi: 10.1038/s41467-023-38909-4. 10.1038/s41467-023-38909-4 PubMed 37270532
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