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Addgene

pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin with the following enzymes: SphI-HF

Expected bands:
For EcoRV-HF, 8125 + 6135 bp
For SphI-HF, 9666 + 4594 bp

Results:
We observe the expected bands for these digests.