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Addgene

pBM4607
(Plasmid #18795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOBD2
  • Backbone size w/o insert (bp) 7167
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sir4
  • Alt name
    STE9
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    891
  • Mutation
    aa 951-1200
  • GenBank ID
    M37249
  • Entrez Gene
    SIR4 (a.k.a. YDR227W, ASD1, STE9, UTH2)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CACAATATTTCAAGCTATACC
  • 3′ sequencing primer CTCATCAACCAACGAAACGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBM4607 was a gift from Mark Johnston (Addgene plasmid # 18795 ; http://n2t.net/addgene:18795 ; RRID:Addgene_18795)

  • For your References section:

    'Calling Cards' method for high-throughput identification of targets of yeast DNA-binding proteins. Wang H, Heinz ME, Crosby SD, Johnston M, Mitra RD. Nat Protoc. 2008;3(10):1569-77. doi: 10.1038/nprot.2008.148. 10.1038/nprot.2008.148 PubMed 18802438
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