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pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
(Plasmid #187936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBdest
  • Backbone manufacturer
    Vienna Biocenter Core Facilities GmbH, Vienna, Austria
  • Backbone size w/o insert (bp) 4958
  • Total vector size (bp) 17204
  • Vector type
    Bacterial Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATG14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2886
  • GenBank ID
    22863
  • Entrez Gene
    ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
  • Tag / Fusion Protein
    • GST-mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GACTCTGCCATGACC
  • 3′ sequencing primer TACCTATGTCCA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    VPS34
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2664
  • GenBank ID
    5289
  • Entrez Gene
    PIK3C3 (a.k.a. VPS34, Vps34, hVps34)

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGTACTACGAGAAGG
  • 3′ sequencing primer GGCCAGAGTAGAGTT
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    VPS15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2664
  • GenBank ID
    30849
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)

Cloning Information for Gene/Insert 3

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGAAGAGGGCTATGG
  • 3′ sequencing primer CAGCGCTCTTGTGTT
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    BECN1
  • Alt name
    beclin1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1353
  • GenBank ID
    8678
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)

Cloning Information for Gene/Insert 4

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGAGGGTTCTAAGACC
  • 3′ sequencing primer TATTTGTTGTAGAACTGCGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Manufactured by the ProTech Facility VBCF Vienna, Austria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 was a gift from Sascha Martens (Addgene plasmid # 187936 ; http://n2t.net/addgene:187936 ; RRID:Addgene_187936)

  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627
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