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Addgene

pCold I-Hero9
(Plasmid #187927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCold I
  • Backbone manufacturer
    Takara
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hero9
  • Alt name
    C9orf16
  • Alt name
    Bublin coiled-coil protein
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • Flag-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also refer to Morimoto et al. (2022) "Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins" bioRxiv. https://doi.org/10.1101/2022.03.03.482928

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCold I-Hero9 was a gift from Yukihide Tomari (Addgene plasmid # 187927 ; http://n2t.net/addgene:187927 ; RRID:Addgene_187927)

  • For your References section:

    A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation. Tsuboyama K, Osaki T, Matsuura-Suzuki E, Kozuka-Hata H, Okada Y, Oyama M, Ikeuchi Y, Iwasaki S, Tomari Y. PLoS Biol. 2020 Mar 12;18(3):e3000632. doi: 10.1371/journal.pbio.3000632. eCollection 2020 Mar. 10.1371/journal.pbio.3000632 PubMed 32163402
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