MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1
(Plasmid
#187925)
-
PurposesgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneMIGRI
-
Vector typeMammalian Expression, Lentiviral, CRISPR
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namehU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBABE5' (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IS1 transposable element is between the two Bbs1 RE sites and will be removed when cloning in a gRNA sequence, and the ccdB selection still works.
Please visit https://doi.org/10.1101/2021.07.21.453193 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1 was a gift from Martin Turner (Addgene plasmid # 187925 ; http://n2t.net/addgene:187925 ; RRID:Addgene_187925) -
For your References section:
A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells. Turner DJ, Saveliev A, Salerno F, Matheson LS, Screen M, Lawson H, Wotherspoon D, Kranc KR, Turner M. eLife. 2022 Apr 22;11. pii: 72313. doi: 10.7554/eLife.72313. 10.7554/eLife.72313 PubMed 35451955
