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pGFP-Tmprss6 (delN)
(Plasmid #18792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1 (Clontech)
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II transmembrane serine protease 6
  • Alt name
    Tmprss6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2276
  • Mutation
    deleted amino acids 1-54
  • Entrez Gene
    Tmprss6 (a.k.a. 1300008A22Rik)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer
  • 3′ sequencing primer EGFP-C Sequencing Primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-Tmprss6 (delN) was a gift from Bruce Beutler (Addgene plasmid # 18792 ; http://n2t.net/addgene:18792 ; RRID:Addgene_18792)

  • For your References section:

    The serine protease TMPRSS6 is required to sense iron deficiency. Du X, She E, Gelbart T, Truksa J, Lee P, Xia Y, Khovananth K, Mudd S, Mann N, Moresco EM, Beutler E, Beutler B. Science. 2008 May 23. 320(5879):1088-92. 10.1126/science.1157121 PubMed 18451267
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