pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008
(Plasmid #187916)

• Purpose: Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells
• Depositing Lab: Arindam Mondal
• Publication: Kedia et al Front Microbiol. 2022 May 25;13:868367. doi: 10.3389/fmicb.2022.868367. eCollection 2022.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5426
• Total vector size (bp): 7814
• Modifications to backbone: C terminal FLAG tag following a tri-Alanine linker
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Streak an ampicillin agar plate from the frozen glycerol stock. Grow bacterial culture from a single colony grown on the plate.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: polymerase basic protein 2
• Alt name: PB2
• Species: B/Brisbane/60/2008
• Insert Size (bp): 2388
• Promoter: CMV Promoter
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry