TFAM-SNAPf
(Plasmid #187909)

• Purpose: Mammalian cell expression of SNAPf-labeled TFAM
• Depositing Lab: Erika Holzbaur
• Publication: Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005.

Backbone

• Vector backbone: pSNAPf
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 6500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TFAM
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NM_003201.3
• Entrez Gene: TFAM (a.k.a. MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3)
• Promoter: CMV
• Tag / Fusion Protein:
  • SNAPf (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: 3' Sequencing Primer: puSNAPf 5'-CTGCATCAGTGGCTCTGGT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TFAM subcloned from Addgene 67064

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.