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Addgene

c-Fos
(Plasmid #187905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7208
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cFos
  • Alt name
    Fos
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1796
  • Entrez Gene
    FOS (a.k.a. AP-1, C-FOS, p55)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloning by Jennifer A Mitchell.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-Fos was a gift from Jennifer Mitchell (Addgene plasmid # 187905 ; http://n2t.net/addgene:187905 ; RRID:Addgene_187905)

  • For your References section:

    Differential activation of the connexin 43 promoter by dimers of activator protein-1 transcription factors in myometrial cells. Mitchell JA, Lye SJ. Endocrinology. 2005 Apr;146(4):2048-54. doi: 10.1210/en.2004-1066. Epub 2004 Dec 23. 10.1210/en.2004-1066 PubMed 15618352
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