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Addgene

p3xFLAG-Tmprss6(Mask)
(Plasmid #18790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV-14 (Sigma)
  • Backbone size w/o insert (bp) 6265
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II transmembrane serine protease 6
  • Alt name
    Tmprss6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1785
  • Mutation
    deleted amino acids 567-811 and replaced with 28 abberant amino acids.
  • Entrez Gene
    Tmprss6 (a.k.a. 1300008A22Rik)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer N-CMV-30 sequencing primer
  • 3′ sequencing primer C-CMV-24 sequencing primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-Tmprss6(Mask) was a gift from Bruce Beutler (Addgene plasmid # 18790 ; http://n2t.net/addgene:18790 ; RRID:Addgene_18790)

  • For your References section:

    The serine protease TMPRSS6 is required to sense iron deficiency. Du X, She E, Gelbart T, Truksa J, Lee P, Xia Y, Khovananth K, Mudd S, Mann N, Moresco EM, Beutler E, Beutler B. Science. 2008 May 23. 320(5879):1088-92. 10.1126/science.1157121 PubMed 18451267
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