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HA-EGFP-NEMO-D304N
(Plasmid #187898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-EGFP-NEMO-D304N
  • Alt name
    IKBKG
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    D304N
  • GenBank ID
    AF069542
  • Entrez Gene
    Ikbkg (a.k.a. 1110037D23Rik, IKK[g], NEMO)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Blp1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pd0.5-EGFP-C 5'-GACCACTACCAGCAGAACAC
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E. Laplantine (Institut Pasteur), Tarantino et al. JCB 2014

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-EGFP-NEMO-D304N was a gift from Erika Holzbaur (Addgene plasmid # 187898 ; http://n2t.net/addgene:187898 ; RRID:Addgene_187898)

  • For your References section:

    Damaged mitochondria recruit the effector NEMO to activate NF-kappaB signaling. Harding O, Holzer E, Riley JF, Martens S, Holzbaur ELF. Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005. 10.1016/j.molcel.2023.08.005 PubMed 37683611
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