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Addgene

Parkin
(Plasmid #187897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5300
  • Modifications to backbone
    mCherry fusion tag was deleted
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKN
  • Alt name
    Park2
  • Alt name
    Parkin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    None
  • GenBank ID
    NM_004562
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Promoter CMV
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Not available
  • 3′ sequencing primer Not available
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R. Youle, NIH

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subcloned from Addgene 23956.
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Parkin was a gift from Erika Holzbaur (Addgene plasmid # 187897 ; http://n2t.net/addgene:187897 ; RRID:Addgene_187897)

  • For your References section:

    Damaged mitochondria recruit the effector NEMO to activate NF-kappaB signaling. Harding O, Holzer E, Riley JF, Martens S, Holzbaur ELF. Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005. 10.1016/j.molcel.2023.08.005 PubMed 37683611
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