CMV mRuby3-Synapsin1a
(Plasmid #187896)

• Purpose: For labeling pre-synaptic terminals
• Depositing Lab: Timothy Ryan
• Publication: Pre-synaptic marker (unpublished)

Backbone

• Vector backbone: pEGFP-C1 vector
• Backbone manufacturer: Clontech, Palo Alto, California
• Backbone size w/o insert (bp): 3998
• Total vector size (bp): 6839
• Modifications to backbone: A plasmid to express mRuby-synapsin was generated by removing GFP from GFP-synapsin (Chi et al., 2001) using restriction sites AgeI and BglII, and substituting it in frame with mRuby obtained from pKanCMV-mRuby3-18aa-actin, which was a gift from Michael Lin (Addgene plasmid # 74255).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synapsin1a
• Species: R. norvegicus (rat)
• Insert Size (bp): 2115
• Promoter: CMV
• Tag / Fusion Protein:
  • mRuby3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • CMV-mRuby3-synapsin.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

F255Y and S304A mutations in Synapsin1a insert do not affect plasmid function.