pCpfSA
(Plasmid
#187890)
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Purpose(Empty Backbone) CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCpfSA was a gift from Quanjiang Ji (Addgene plasmid # 187890 ; http://n2t.net/addgene:187890 ; RRID:Addgene_187890) -
For your References section:
CRISPR/Cpf1-Mediated Multiplex and Large-Fragment Gene Editing in Staphylococcus aureus. Wang Z, Wang Y, Wang Y, Chen W, Ji Q. ACS Synth Biol. 2022 Sep 16;11(9):3049-3057. doi: 10.1021/acssynbio.2c00248. Epub 2022 Aug 24. 10.1021/acssynbio.2c00248 PubMed 36001082