p3xFLAG-Tmprss6
(Plasmid #18789)

• Depositing Lab: Bruce Beutler
• Publication: Du et al Science. 2008 May 23. 320(5879):1088-92.

Backbone

• Vector backbone: p3xFLAG-CMV-14
• Backbone size w/o insert (bp): 6265
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Type II transmembrane serine protease 6
• Alt name: Tmprss6
• Species: M. musculus (mouse)
• Insert Size (bp): 2436
• Entrez Gene: Tmprss6 (a.k.a. 1300008A22Rik)
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: N-CMV-30 sequencing primer
• 3′ sequencing primer: C-CMV-24 sequencing primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry