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Addgene

pAcGP67A-mMD2-proA
(Plasmid #187878)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGP67a
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MD2
  • Species
    M. musculus (mouse)
  • Mutation
    includes residues 19–160 of mouse MD-2
  • Entrez Gene
    Ly96 (a.k.a. ESOP-1, MD-2, MD2)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A-mMD2-proA was a gift from Bruce Beutler (Addgene plasmid # 187878 ; http://n2t.net/addgene:187878 ; RRID:Addgene_187878)

  • For your References section:

    TLR4/MD-2 activation by a synthetic agonist with no similarity to LPS. Wang Y, Su L, Morin MD, Jones BT, Whitby LR, Surakattula MM, Huang H, Shi H, Choi JH, Wang KW, Moresco EM, Berger M, Zhan X, Zhang H, Boger DL, Beutler B. Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E884-93. doi: 10.1073/pnas.1525639113. Epub 2016 Feb 1. 10.1073/pnas.1525639113 PubMed 26831104
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