pEA186
(Plasmid
#187874)
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Purpose(Empty Backbone) Empty pVS1-based INTEGRATE vector with mCherry cargo
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemodified pTF505
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Backbone manufacturerKeunsub Lee
- Backbone size (bp) 6149
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Modifications to backbonePoint mutation was introduced to destroy BsaI restriction site.
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Vector typeBacterial Expression, CRISPR
- Promoter J23119
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CAAAGTGTCTcCGCGTTCTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEA186 was a gift from Kan Wang (Addgene plasmid # 187874 ; http://n2t.net/addgene:187874 ; RRID:Addgene_187874) -
For your References section:
CRISPR RNA-guided integrase enables high-efficiency targeted genome engineering in Agrobacterium tumefaciens. Aliu E, Lee K, Wang K. Plant Biotechnol J. 2022 Jun 11. doi: 10.1111/pbi.13872. 10.1111/pbi.13872 PubMed 35690588
