pTBS 5Y YIC
(Plasmid #18786)

• Depositing Lab: Han Htun
• Publication: Nie et al Nucleic Acids Res. 2006 . 34(19):5528-40.

Backbone

• Vector backbone: pYIC
• Backbone manufacturer: Available at Addgene (#18673)
• Backbone size w/o insert (bp): 6270
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRAP binding site
• Alt name: TBS
• Insert Size (bp): 55

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pTBS 5Y YIC
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).