pMN cFKT
(Plasmid #18785)

• Depositing Lab: Han Htun
• Publication: Nie et al Nucleic Acids Res. 2006 . 34(19):5528-40.

Backbone

• Vector backbone: n/a
• Backbone size w/o insert (bp): 4200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRAP
• Alt name: tryptophan RNA-binding attenuation protein
• Alt name: mtrB
• Species: Bacillus subtilis
• Insert Size (bp): 330
• Entrez Gene: mtrB
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pMN cFKT
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mammalian expression vector for the expression of HA and His6 epitope-tagged TRAP. There is a single amino acid (glycine) insertion between translational initiator codon and second amino acid of TRAP (asparagine) to maintain Kozak consensus sequence. Also, there is a single amino acid difference at amino acid #60 which has an asparagine (N) as in Bacillus amyloliquefaciens rather than glutamic acid (E) as in Bacillus subtilis. Codons are a mixture of human, Bacillus amyloliquefaciens, and Bacillus subtilis.