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Addgene

pVoPo-GFP
(Plasmid #187840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVoPo-00
  • Backbone size w/o insert (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Alt name
    sfGFP codon optimized for F. nucleatum
  • Species
    Synthetic
  • Insert Size (bp)
    711

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains an IS4-like element inserted at the end of ColE1. This is not expected to impact plasmid function. Please choose green fluorescent colonies when selecting for growth.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVoPo-GFP was a gift from Jörg Vogel (Addgene plasmid # 187840 ; http://n2t.net/addgene:187840 ; RRID:Addgene_187840)

  • For your References section:

    Expanding the genetic toolkit helps dissect a global stress response in the early-branching species Fusobacterium nucleatum. Ponath F, Zhu Y, Cosi V, Vogel J. Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 10.1073/pnas.2201460119. Epub 2022 Sep 26. 10.1073/pnas.2201460119 PubMed 36161895
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