pVoPo-GFP
(Plasmid
#187840)
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PurposeConstitutive GFP-expression vector for Fusobacterium nucleatum
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVoPo-00
- Backbone size w/o insert (bp) 3600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP
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Alt namesfGFP codon optimized for F. nucleatum
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SpeciesSynthetic
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Insert Size (bp)711
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains an IS4-like element inserted at the end of ColE1. This is not expected to impact plasmid function. Please choose green fluorescent colonies when selecting for growth.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVoPo-GFP was a gift from Jörg Vogel (Addgene plasmid # 187840 ; http://n2t.net/addgene:187840 ; RRID:Addgene_187840) -
For your References section:
Expanding the genetic toolkit helps dissect a global stress response in the early-branching species Fusobacterium nucleatum. Ponath F, Zhu Y, Cosi V, Vogel J. Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 10.1073/pnas.2201460119. Epub 2022 Sep 26. 10.1073/pnas.2201460119 PubMed 36161895
Map uploaded by the depositor.
