Skip to main content
Addgene

pEcoFus
(Plasmid #187833)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRPF185
  • Backbone size (bp) 9035
  • Modifications to backbone
    Replacement of origin of replication for Clostridium difficile with that for Fusobacterium nucleatum. Replacement of tetR-gus cassette with constitutive fusobacterial promoter (4.5S RNA)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The fusobacterial origin of replication was transferred from pROI92 provided by G. Bachrach (PMID: 15574887)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEcoFus was a gift from Jörg Vogel (Addgene plasmid # 187833 ; http://n2t.net/addgene:187833 ; RRID:Addgene_187833)

  • For your References section:

    RNA landscape of the emerging cancer-associated microbe Fusobacterium nucleatum. Ponath F, Tawk C, Zhu Y, Barquist L, Faber F, Vogel J. Nat Microbiol. 2021 Aug;6(8):1007-1020. doi: 10.1038/s41564-021-00927-7. Epub 2021 Jul 8. 10.1038/s41564-021-00927-7 PubMed 34239075
Related items:
Commonly requested with: