GST-3C-FIP200-GFP
(Plasmid #187832)

• Purpose: Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445
• Depositing Lab: Sascha Martens
• Publication: Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi: 10.1038/s41467-021-25572-w.

Backbone

• Vector backbone: pGB-02-03
• Backbone manufacturer: Protech Facilities VBCF, Vienna, Austria
• Backbone size w/o insert (bp): 2935
• Total vector size (bp): 9145
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FIP200
• Alt name: ATG17
• Species: H. sapiens (human)
• Insert Size (bp): 6210
• Entrez Gene: RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • mGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: acggatcCcggtccgaaaccatgtcccctatactaggt
• 3′ sequencing primer: TCCCCAGAACATCAGGTTAATGGCGttacttg

Resource Information

• Supplemental Documents:
  • SMC1445-631_S_0_1421_0SM001-GST-FIP200-GFP.str.txt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry