Skip to main content
Addgene

pFastBac_Dual_GST-TEV-EGFP-TBK1
(Plasmid #187830)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBac_Dual
  • Backbone manufacturer
    Thermofisher Scientific
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 8776
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TBK1
  • Alt name
    NAK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3625
  • GenBank ID
    29110 NM_013254
  • Entrez Gene
    TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • TEV-mEGFP-GG Linker (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGAGGCATGCAATCCACATCCAACCACC
  • 3′ sequencing primer CCTCTAGTACTTCTCGACAAGCTTTTACAGACAGTCCACGTTTCTCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon Optimized for Sf9 Expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac_Dual_GST-TEV-EGFP-TBK1 was a gift from Sascha Martens (Addgene plasmid # 187830 ; http://n2t.net/addgene:187830 ; RRID:Addgene_187830)

  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627
Related items:
Commonly requested with: