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pGST2-GST-TEV-OPTN S177D S473D
(Plasmid #187827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST2
  • Backbone manufacturer
    James Hurley Lab
  • Backbone size w/o insert (bp) 4905
  • Total vector size (bp) 6694
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OPTN
  • Alt name
    GLC1E
  • Species
    H. sapiens (human)
  • Mutation
    S177D S473D
  • GenBank ID
    10133 NM_021980
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
  • Tag / Fusion Protein
    • GST-TEV (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CATGTCCCCTATACTAGGTTATTGGAAAAT
  • 3′ sequencing primer TTAaatgatgcaatccatcacgtgaatctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-GST-TEV-OPTN S177D S473D was a gift from Sascha Martens (Addgene plasmid # 187827 ; http://n2t.net/addgene:187827 ; RRID:Addgene_187827)

  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627
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