Skip to main content
Addgene

pET28a-nGFPMNase
(Plasmid #187826)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6166
  • Vector type
    Bacterial Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nGFPMNASE
  • Species
    Synthetic
  • Insert Size (bp)
    852
  • Promoter T7 Promotor
  • Tag / Fusion Protein
    • his tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAGCGGATAACAATTCCCCT
  • 3′ sequencing primer AGGCCCCAAGGGGTTATGCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-nGFPMNase was a gift from Yikang Rong (Addgene plasmid # 187826 ; http://n2t.net/addgene:187826 ; RRID:Addgene_187826)

  • For your References section:

    Elevation of D4 dopamine receptor mRNA in postmortem schizophrenic brain. Stefanis NC, Bresnick JN, Kerwin RW, Schofield WN, McAllister G. Brain Res Mol Brain Res. 1998 Jan;53(1-2):112-9. doi: 10.1016/s0169-328x(97)00285-4. 10.1016/s0169-328x(97)00285-4 PubMed 9473618
Related items: