UAS:TVA-mCherry
(Plasmid #187823)

• Purpose: Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.
• Depositing Lab: Rainer Friedrich
• Publication: Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi: 10.7554/eLife.77153.

Backbone

• Vector backbone: 5xUAS Tol2
• Backbone size w/o insert (bp): 3706
• Total vector size (bp): 5034
• Modifications to backbone: bGH added to Tol2 vector by Shin-ichi Higashijima. (5xUAS Tol2 vectors as in Asakawa, K. et al. Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Proc. Natl. Acad. Sci. U. S. A. 105, 1255–1260 (2008).)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TVA-mCherry
• Insert Size (bp): 1680
• GenBank ID: AY531260.1
• Promoter: 5xUAS

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • bGH poly(A) signal.gb
  • tol2_backbone.gb
  • TVA-mCherry.gb
  • UAS_E1b.gb
• A portion of this plasmid was derived from a plasmid made by: Chie Satou, created it in the Rainer Friedrich's lab (FMI-Basel) and asked Estelle Arn (lab manager) to deposite it at Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2021.03.25.436574 for bioRxiv preprint.

DNA construction: TVA-mCherry was amplified by PCR from a plasmid (gift from Dr. Uchida, Addgene plasmid #38044 ; http://n2t.net/addgene:38044 ; RRID:Addgene_38044) and inserted into a 5xUAS vector (Asakawa, K. et al. Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Proc. Natl. Acad. Sci. U. S. A. 105, 1255–1260 (2008).).

Used to create transgenic Zebrafish and make use of the Gal4/UAS system.

Note: Addgene's NGS results identified several discrepancies with the depositor's in silico generated reference sequences. The depositing lab has confirmed the discrepancies and confirmed that the plasmid functions described in the associated publication.