GB3897
(Plasmid
#187809)
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PurposeModule for dCasEV2.1 activated expression of AtrD11, HarFAR and ScATF1 plus gRNA-1DFR.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3_alpha2
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMoth Pheromone pathway
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the Transcriptional Units for constitutive expression of kanamycin resistance and gRNA1 (pDFR), and for dCasEV2.1-regulated expression of ATF1, HarFAR and Atr delta11
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB3897 was a gift from Diego Orzaez (Addgene plasmid # 187809 ; http://n2t.net/addgene:187809 ; RRID:Addgene_187809) -
For your References section:
Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N. Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. 10.1111/pbi.14048 PubMed 37032497