GB3682
(Plasmid
#187807)
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PurposeTranscriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces pastorianus strain CBS 1483 chromosome SeVIII-SeXV, codon optimized for Nicotiana.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3_alpha2
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpATF1-2
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB3682 was a gift from Diego Orzaez (Addgene plasmid # 187807 ; http://n2t.net/addgene:187807 ; RRID:Addgene_187807) -
For your References section:
Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N. Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. 10.1111/pbi.14048 PubMed 37032497