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Addgene

Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996
(Plasmid #187732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA
  • Alt name
    Q14524
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1014
  • Mutation
    deletion of residues 1-410 and 718-1959 and 1997-2016; residues 1974-1977 PPSY mutated to AASA
  • Entrez Gene
    SCN5A (a.k.a. CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1)
  • Tag / Fusion Protein
    • His-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996 was a gift from Sandra Gabelli (Addgene plasmid # 187732 ; http://n2t.net/addgene:187732 ; RRID:Addgene_187732)

  • For your References section:

    NEDD4L intramolecular interactions regulate its auto and substrate Na(V)1.5 ubiquitination. Wright KM, Nathan S, Jiang H, Xia W, Kim H, Chakouri N, Nwafor JN, Fossier L, Srinivasan L, Chen Z, Boronina T, Post J, Paul S, Cole RN, Ben-Johny M, Cole PA, Gabelli SB. J Biol Chem. 2024 Mar;300(3):105715. doi: 10.1016/j.jbc.2024.105715. Epub 2024 Feb 2. 10.1016/j.jbc.2024.105715 PubMed 38309503
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