pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
(Plasmid
#187723)
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PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBacHT-A
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNEDD4L residues 1-418+582-975
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Alt nameQ96PU5
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SpeciesH. sapiens (human)
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Insert Size (bp)2511
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Mutationdeletion of residues 419-581
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Entrez GeneNEDD4L (a.k.a. NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2)
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Tag
/ Fusion Protein
- His-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT was a gift from Sandra Gabelli (Addgene plasmid # 187723 ; http://n2t.net/addgene:187723 ; RRID:Addgene_187723) -
For your References section:
NEDD4L intramolecular interactions regulate its auto and substrate Na(V)1.5 ubiquitination. Wright KM, Nathan S, Jiang H, Xia W, Kim H, Chakouri N, Nwafor JN, Fossier L, Srinivasan L, Chen Z, Boronina T, Post J, Paul S, Cole RN, Ben-Johny M, Cole PA, Gabelli SB. J Biol Chem. 2024 Mar;300(3):105715. doi: 10.1016/j.jbc.2024.105715. Epub 2024 Feb 2. 10.1016/j.jbc.2024.105715 PubMed 38309503