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pET28a(+)-NEDD4L_W3-W4-HECT_C874only
(Plasmid #187711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NEDD4L residues 492-975 with mutations
  • Alt name
    Q96PU5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1452
  • Mutation
    deletion of residues 1-491; mutations C702A, C776S, C853A, C874S for chemical biology
  • Entrez Gene
    NEDD4L (a.k.a. NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2)
  • Tag / Fusion Protein
    • His-HRV 3C site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)-NEDD4L_W3-W4-HECT_C874only was a gift from Sandra Gabelli (Addgene plasmid # 187711 ; http://n2t.net/addgene:187711 ; RRID:Addgene_187711)

  • For your References section:

    NEDD4L intramolecular interactions regulate its auto and substrate Na(V)1.5 ubiquitination. Wright KM, Nathan S, Jiang H, Xia W, Kim H, Chakouri N, Nwafor JN, Fossier L, Srinivasan L, Chen Z, Boronina T, Post J, Paul S, Cole RN, Ben-Johny M, Cole PA, Gabelli SB. J Biol Chem. 2024 Mar;300(3):105715. doi: 10.1016/j.jbc.2024.105715. Epub 2024 Feb 2. 10.1016/j.jbc.2024.105715 PubMed 38309503
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