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Addgene

Cdk4-DN-HA Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 1877 with XhoI and SpeI-HF

We expected and saw the following banding pattern:

XhoI / SpeI-HF double digest: 2 bands: 5.4 and 2 kb