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Addgene

pLenti-Zyxin-B (aa309-564)-EGFP
(Plasmid #187694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zyxin
  • Species
    M. musculus (mouse)
  • Mutation
    aa309-564
  • Entrez Gene
    Zyx (a.k.a. 9530098H06Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Zyxin-B (aa309-564)-EGFP was a gift from Mary Beckerle (Addgene plasmid # 187694 ; http://n2t.net/addgene:187694 ; RRID:Addgene_187694)

  • For your References section:

    Stretch-induced actin remodeling requires targeting of zyxin to stress fibers and recruitment of actin regulators. Hoffman LM, Jensen CC, Chaturvedi A, Yoshigi M, Beckerle MC. Mol Biol Cell. 2012 May;23(10):1846-59. doi: 10.1091/mbc.E11-12-1057. Epub 2012 Mar 28. 10.1091/mbc.E11-12-1057 PubMed 22456508
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