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pLenti-Lifeact EGFP
(Plasmid #187686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    51
  • Mutation
    WT
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lifeact sequence removed from vector backbone and replaced with gene insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Lifeact EGFP was a gift from Mary Beckerle (Addgene plasmid # 187686 ; http://n2t.net/addgene:187686 ; RRID:Addgene_187686)

  • For your References section:

    LIM domains target actin regulators paxillin and zyxin to sites of stress fiber strain. Smith MA, Blankman E, Deakin NO, Hoffman LM, Jensen CC, Turner CE, Beckerle MC. PLoS One. 2013 Aug 21;8(8):e69378. doi: 10.1371/journal.pone.0069378. eCollection 2013. 10.1371/journal.pone.0069378 PubMed 23990882
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